High Quality of Ponceau S CAS 6226-79-5 C. I. 27195 4-Sulfophenylazo

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Model NO.	SUREST-119	Content	Standard
Usage	Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents	Source	Dry Powder
Habit Appellation	Special Reagent	Application	Industry, Scientific Research, Health, Environmental Protection, Agriculture
Property	Organic Reagent	Shelf Life	2 Years
Stock	Available	Molecular Weight	760.57
Color	Bordeaux Red	Transport Package	25kg /Drum
Specification	98%min	Trademark	Surest
Origin	China	HS Code	3204901000
Production Capacity	1000 Tons Yearly

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Product Description

Ponceau S

Name	Ponceau S
Synonyms	C.I. 27195; 3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid tetrasodium salt

Molecular Structure
Molecular Formula	C22H12N4Na4O13S4
Molecular Weight	760.57
CAS Registry Number	6226-79-5
EINECS	228-319-2

Properties Water solubility 10 g/L

Chemical Property/Description

Bordeaux red to dark red or brown powder,Ponceau S is negatively charged and can bind to positively charged amino acid residues, while ponceau can also bind to non-polar regions of proteins to form red bands. The staining of proteins by Ponceau S is reversible and can be washed away with distilled water, PBS, or other appropriate solutions.

Main Uses:

Biological staining, protein staining after electrophoresis. Ponceau S can be used to detect proteins attached to NC and PVDF membranes, but cannot be used for Nylon membranes.

Determination of Cu in food by ponceau S probe dual wavelength absorption spectrometry. In TRIS-hydrochloric acid buffer medium at pH 6.86, ponceumin S reacts with Cu(2) to form an ion association with an obvious positive absorption peak and a clear negative absorption peak in the visible region. The positive and negative absorption wavelengths are 445 nm and 510 nm, respectively. Cu(2) follows Lambert-Biers law in 0.06~1.6 mg/L(positive absorption, 445 nm), 0.04~1.6 mg/L(negative absorption, 510 nm) and 0.03~1.6 mg/L(DWO-VIS, 445 nm+510 nm). The apparent molar absorptivities (κ) were 8.70× 10-3 (positive absorption), 2.04× 10-4 (negative absorption) and 2.92× 10-4 (DWO-VIS)L/(mol·cm), respectively. The limits of quantitation were 0.62, 0.38 and 0.26 mg/100 g, respectively. The effects of absorption spectra, suitable reaction conditions and co-existing substances were also discussed. The dual-wavelength method was used to determine Cu in biscuits, noodles and milk powder. The recoveries were 97.9%-102% and the relative standard deviations (RSD) were 2.5%-2.8% (n=5).